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Laboratory of Nitrogen Fixation and Assimilation Enzymes

Head of laboratory, Doctor, Professor T.Sadunishvili

Contacts

Phone/Fax: (995 32) 52 0618
Ĺ-mail: sadunishvili_tinatin@yahoo.com

Main Directions

Creation and study of free living nitrogen-fixing microorganisms and actinomycetes collection.
Study of nitrogen fixing and assimilating enzymes (glutamine synthetase, glutamate dehydrogenase) physical - chemical properties, activity, and synthesis regulation pathways and function in higher plants (leguminous cultures: soy, kidney bean, cereal cultures: maize, grasses ryegrass).
Study of plant structural and functional changes under different stress conditions including envioronment polluted by heavy metals and organic toxicants.
Creation of biological fertilizers enriched by nitrogen fixations.
Elaboration of the methods for biological control of plant bacteriosis by using bacteriophage.

Research Activity

The collection of actinomycetes, from strains isolated from various soil-climatic zones of Georgia has been created. Actinomycetes capability to produce various hydrolyzing enzymes and degrade environmental organic pollutants has been established.
Nitrogen assimalation pathways have been established, enzymes, participating in nitrogen fixation and assimilation processes and regulatory pathways of their action in free living nitrogen-fixing microorganisms and higher plants.
The role of enzymes (glutamine synthetase, glutamate dehydrogenase) participating in nitrogen assimilation during detoxification of organic toxicants has been revealed.
By the action of microorganisms several types of biological fertilizers have been obtained from such natural formation as sapropeli.
Bacteriophage against tomato bacterial black spot has been obtained.

Members

  1. Sadunishvili Tinatin - Doctor, professor, head of laboratory
  2. Nutsubidze Nodar – Doctor, Academician, chief scientist
  3. Pataraya Dodo - Doctor, chief scientist
  4. Zaalishvili Giorgi – Doctor, senior scientist
  5. Amashukeli Nanuli – PhD scientist
  6. Gaganidze Dali – PhD, scientist
  7. Betsiashvili Mariam – PhD, scientist
  8. Gurielidze Manana - PhD, scientist
  9. Kuprava Nana – PhD, Lab assistant
  10. Ghudumidze Nino - Lab assistant
  11. Shapovalova Nino - Lab assistant
  12. Dzamukashvili Nana - Lab assistant

Sadunishvili Tinatin

1952– Born
1975 - Tbilisi State University.
1980 - Candidate of Biological Sciences. Moscow.
1983 - Bach institute of Biochemistry, Academy of Sciences of USSR.
1987 - Chavchavadze Institute of Foreign Languages. Highest Courses of Simultaneous  Interpreters.
1987 - Etvos Lorand University, Budapest, Hungary. Scientific trainee.
1997-1988 Guest Scientist. Institute of Enzymology, Szegedi Biological Centre of the Hungarian Academy of Sciences, Hungary.
1991 - Scientific degree – Senior Scientist. Moscow.
1993 - Professor of Biotechnology, Georgian Technical University, Department of    Biotechnology, Tbilisi
1995 - Doctor of Biological sciences.
1995 - International Course on Food Technology (Subjects: Biotechnology, Food Microbiology, Grain Storage, Quality Assuarence). Hebrew University of Jerusalem State of Israel. Certificate.
2004-2007 - Trainings in LBNL, Berkeley, CA, Diversa, San-Diego, CA, INRA, Toulouse, France, etc.
2003 - Head of laboratory

Selected Publications

  1. Sadunishvili T.A., Nutsubidze, N.N. The influence of ammonia on glutamine synthetase and glutamate dehydrogenase in vivo and their intracellular localization in kidney bean leaves. Biokhimiya, 1985, 50, 3, 820-825. (In Russian).
  2. Sadunishvili T.A., Nutsubidze, N.N. Multiple molecular forms of glutamate dehydrogenase in kidney bean. Prikl. Biokhim. Mikrobiol., 1986, 22, 337-340 (In Russian).
  3. Sadunishvili T.A., Gvarliani, N.Z., Maziashvili, M.I., Nutsubidze, N.N. Data on ammonia assimilation in kidney bean leaves through glutamin. Bulletin of the Georgian Academy of Sciences, 1989, 133, 3, 637-640. (In Russian).
  4. Sadunishvili T.A., Gvarliani N.Z., Nutsubidze N.N. Primary assimilation of ammonia in kidney bean leaves. Biochemistry (Moscow), 1989, 3, 397-401.
  5. Sadunishvili T., Gvarliani, M. Nutsubidze, N., Kvesitadze, G. Enzymatic mechanism of ammonia excess detoxication in kidney bean. Fresenius Environmental Bulletin, 1993, 2, 534-539.
  6. Kvesitadze G.I., Kokonashvili, G.N., Sadunishvili, T.A. Enzymes of nitrogen and energy metabolism from the liver of spiny dogfish and in the preparation Katrex. Applied Biochemistry and Microbiology, 1993, 29, 1, 102-106.
  7. Sadunishvili T., Nutsubidze, N., Kvesitadze, G. Effect of methionine sulfoximine on nitrogen metabolism and externally supplied ammonium assimilation in Kidney bean. Ecotoxicol. Environ. Safety, 1996, 34, 70-75.
  8. Sadunishvili T. Effects of light, nitrate and ammonium on bean ferredoxin- and NADH-dependent glutamate synthases. Applied Biochemistry and Microbiology, 1996, 32, 2, 231-233.
  9. Sadunishvilii T. Organ-specific distribution and subcellular localization of ferredoxin- and NAD-specific glutamate synthases in kidney bean. Fiziologiya i Biokhimia Kulturnikh Rastenii, 1997, 29, 2, 133-138(In Russian).
  10. Sadunishvili T., Gvarliani N., Nutsubidze N. The pathway of ammonium primary assimilation in kidney bean. Turkish Journal of Agriculture and Forestry, 1997, 21, 551-556.
  11. Sadunishvilii T. Metabolic pathways of ammonium assimilation in kidney bean roots. Fiziologiya i Biokhimia Kulturnikh Rastenii, 2000, 32, 1, 35-40.
  12. Buadze O., Sadunishvili, T., Kvesitadze, G. The effect of 1,2-benzantracene and 3,4-benzpyrene on the ultrastructure of maize cells. International Biodeterioration and Biodegradation, 1998, 41, 119-125.
  13. Zaalishvili G., Lomidze, E., Buadze, O., Sadunishvili, T. Tkhelidze, P., Kvesitadze, G. Electron microscopic investigation of benzidine effect on maize root tip cells ultrastructure, DNA synthesis and calcium homeostasis. International Biodeterioration and Biodegradatio,. 2000, 46, 2, 133-140.
  14. Kvesitadze G., Gordeziani M., Khatisashvili G., Sadunishvili T., Ramsden J.J. Review: Some aspects of the enzymatic basis of phytoremediation. Journal of Biological Physics and Chemistry, 2001, 1, 2, 49-57.
  15. Zaalishvili G., Sadunishvili, T., Scalla, R. Laurent, F. and Kvesitadze, G. Electron Microscopic Investigation of Nitrobenzene Distribution and Effect on Plant Root Tip Cells Ultrastructure. Ecotoxicol. Environ. Safety, 2002, 52, 3, 190-197.
  16. Betsiashvili M., Sadunishvili T., Gigolashvili G., Nutsubidze N., Kvesitadze G. Valuable food protein preparation from soybean. Advances in Food Sciences, 2002, 24, 1, 20-23.
  17. Omiadze N., Parlar H., Leupold G., Mchedlishvili N., Gulua L. Akhvlediani K., Abutidze M., Sadunishvili T., Rodriguez-Lopez J.N., Kvesitadze G. Inhibition of apple phenoloxidase and peroxidase by phenolics of tea leaves. Advances in Food Sciences, 2004, 26, 1, 26-31.
  18. Mchedlishvili N., Zamtaradze R., Sadunishvili T., Omiadze N., Gulua L. Effect of different inhibitors on phenoloxidase activity from green husk of walnut (Juglans regia L.). Proceedings of the Georgian Academy of Sciences, 2004, 2, 1-2, 16-19.
  19. Gogniashvili M., Kolot M., Sadunishvili T. Localization and mechanism of action of the ColA plasmid DNA region determining its stable inheritance. Proceedings of the Georgian Academy of Sciences, 2004, 2, 3-4, 8-13.
  20. Alyabyev A., Gordon L., Loseva N., Rachimova G., Tribunskih V., Estrina R., Sadunishvili T., Gulua L., Mchedlishvili N., Rodriguez-Lopez J.N.. A natural inhibitor isolated from tea leaves and its effect on energy processes in model plants. Thermochimica Acta, 2004, 422,109-113.
  21. Lomsianidze I., Sadunishvili T., Dzamukashvili N., Nutsubidze N. Effect of systematic introduction of ammonium nitrate on properties of Ajameti podzolic soil. Proceedings of the Georgian Academy of Sciences,  2005, 3, N 1, 11-16.
  22. Lomsianidze I., Amashukeli N., Sadunishvili T. The effect of ammonium nitrate doses on amino acid content of proteins in maize grains. Bulletin of the Georgian Acaemy of Sciences, 2005, 171, 1, 145-147.
  23. Lomsianidze I., Amashukeli N., Sadunishvili T. Effect of the introduction of different doses of ammonium nitrate in podzolic soil on maize var. Ajametis tetri seed protein content. . Proceedings of the Georgian Academy of Sciences, 2005, 3, 2, 16-20.
  24. Mchedlishvili N., Omiadze N., Gulua L, Abutidze M., Sadunishvili T., Zamtaradze R, Kvesitadze G., & Bendeliani E. Thermostability of plant phenoloxidase and Peroxidase Determining the Technology of their Use in Food Industry. Applied Biochemistry and Microbiology, 2005, 41, 2, 165-170.
  25. Mitaishvili T., Scalla R., Ugrekhelidze D., Tsereteli B., Sadunishvili T., Kvesitadze G. Transformation of aromatic compounds in plants grown under aseptic conditions. Zeitsschrift fur Naturforschung, 2005, 60 97-102.
  26. Best Elly P.H., Kvesitadze G., Khatisashvili G., Sadunishvili T. Plant processes important for the transformation and degradation of explosives contaminants.Zeitsschrift fur Naturforschung, 2005, 60, 340-348.
  27. Kvesitadze G., Khatisashvili G., Sadunishvili T., Evstigneeva ZG. Detoxification of antropogenic toxicants in higher plants. 2005. 216  Maik-Nauka, (In Russian).
  28. Hidaka H. Sadunishvili T., Ramsden J., Aplakov V., Kvesitadze G. Environmental contamination and phytoremediation technologies. Annals of Agrarian Science, 2006, 3, 4, 9-21.
  29. Sadunishvili T., Kuprava N., Aplakov V., Betsiashvili M., Zaalishvili G., Mithaishvili T., Kvesitadze G. Effect of nitrobenzene on ammonia assimilation enzymes and cell ultrastructure in maize and soybean. . Proceedings of the Georgian Academy of Sciences, 2006, 4, 2, 16-22.
  30. Navaro Peran E., Cabezas-Herrera J., Hiner A.N.P., Sadunishvili T. Garcia-Canovas F., Rodrigues-Lopez J.N. Kinetics of the inhibition of bovine liver dihydrofolate reductase by tea catechins: origin of slow-binding inhibition and ph studies. Biochemistry,2005, 44, 20 ,7512 -7525.
  31. Chrikishvili D., Sadunishvili T., Zaalishvili G. Benzoic acid conjugation and the final fate of conjugates in higher plants. Ecotoxicol. Environ. Safety, 2006, 64, 3, 390-399.

Monographs

  1. Kvesitadze G. I., Khatisashvili G.A., Sadunishvili T.A., Evstigneeva Z.G. Metabolism of Anthropogenic Toxicants in Higher Plants. Maik Nauka, Moscow. 2005. 208.
  2.  Kvesitadze G., Khatisashvili G., Sadunishvili T., Ramsden J-J., Biochemical Mechanisms of Detoxification in Higher Plants-Basis of Phytoremediation. Springer, 2006,263 .

Grants of the Georgian Academy of Sciences

  1. 1997–1999 – 14.2. Establishment of nitrogen assimilatory enzyme systems and pathways of protein production regulation in leguminous plants
  2. 2000–2001 – 14.2. Effect of nitrogenous microbiological combined fertilizers on regulatory mechanism of nitrogen assimilation processes in leguminous and cereal cultures and growth – development stimulation of plant.
  3. 2002–2003 – 14.7. Correlation between nitrogen – fixing and nitrogen assimilating processes in symbiotic system of leguminous plants

National Grant

2006 – 2009 – GNSF /ST06/5 – 077 Isolation, characterization of extremophilic actinomycetes from soils of Georgia, the pathways of their application in modern biotechnology.

International Grants

  1. 1998-2002 97-0716 INTAS-Georgia. Coordination of Plant Oxidative Enzymes as a Key Factor in Degradation of Organic Xenobiotics ( http://www.intas.be/mainfs.htm)
  2. 2001-2004. INTAS-FOOD. Prevention of food spoilage by suppression of phenoloxidase, peroxidase and growth of pathogenic microflora by use of natural inhibitors of plant origin, ( http://www.intas.be/mainfs.htm)
  3. 2001-2003 ISTC, G-369. Elaboration of Methods of Bioremediation of Contaminated Soils on Former Military Locations and Proving Grounds in Georgia (http.//www. istc.ru/)
  4.  2002-2005. ISTC G-718. Elaboration of a new strategy of phytoremediation and long-term protection of the environment polluted by hydrocarbons (http.//www. istc.ru/)
  5. 2004-2006. STCU P-196. Microbial diversity for novel biotechnology applications(http.//www. stcu.int)
  6.  2005-2008. ISTC G-1129. Bacteriophage, an effective biological tool against plant diseases caused by pathogenic bacteria. (http.//www. istc.ru/)
  7. 2006-2008. CRDF_SCCRP, SCI-010002-SC-05Establishment of a Biotechnological Network of Regional Microbial Culture Collections in the Caucasus
  8. 2006-2009. STCU 3802. Novel approach for the improvement of ecological guarantee of oil pipelines. (http.//www.stcu.int) 

Nutsubidze Nodar

1929 – Born
1952 - Georgian Agriculture University
1956 - Candidate of Biological Sciences
1970 – Doctor of Biological Sciences
1965–2006 - Head of Laboratory
1979 - Corresponding - member of the Georgian Academy of Sciences
2001 - Academician

Publications

over 260 scientific papers, monography, 3 books

Selected Publications

  1. Sadunishvili T., Gvarliani N., Nutsubidze N. The pathway of ammonium primary assimilation in kidney bean. TurkishJournalofAgricultureandForestry, 1997, 21, 551-556.
  2. Pataraya D., Basilashvili L., Bagalishvili M., Kikvidze M., Betsiashvili M., Nutsubidze N. Influence of nodule bacteria and different microorganisms on soybean growth. Bulletin of the Georgian Academy of Sciences,1999, 160, 2, á334-336.
  3.  Jinoria K., Nutsubidze N., “pH-dependent conformational changes in proteins”. Bulletin of the Georgian Academy of Sciences, 2000, 3-4 , 88-92.
  4.  Sadunishvili,T., Betsiashvili, M., Kuprava, N., Nutsubidze, N., Kvesitadze,G. Preparation of valuable food protein and amino acid mixtures from soybean. Biotechnology, 2000, 3, 226-228.
  5. Pataraya D., Gurielidze M., Nutsubidze N. Biological methods of activity determination of antibiotic compounds produced by actinomycetales. Bulletin of the Georgian Academy of Sciences, 2000, 161, 3, 535-537.
  6. Basilashvili L., Kikvidze M., Bagalishvili M., Nutsubidze N., Effect of Azosporillum on the growth and supply with nitrogen of Zea mays. Bulletin of the Georgian Academy of Sciences, 2001, 163, 1, 167-170.
  7. Basilashvili L., Bagalishvili M., Kikvidze M. Nutsubidze N. Influence of Azospirillum brazilence on Soybean and nodule bacteria Symbioses. Bulletin of the Georgian Academy of Sciences, 2002, 166, 1, 124.
  8. Betsiashvili M., Sadunishvili T., Gigolashvili G., Nutsubidze N., Kvesitadze G. Valuable food protein preparation from soybean. Advances in Food Sciences, 2002, 24, 1, 20-23.
  9.  Betsiashvili, M., Kuprava, N., Sadunishvili, T., Nutsubidze, N. Study of the Georgian Soybean Varieties for Preparation of Valuable Food Protein. Bulletin of the Georgian Academy of Sciences, 2002, 165, 2, 358-360.
  10.  Betsiashvili,M., Kuprava, N., Sadunishvili, T., Nutsubidze, N. Study of the Soybean Protein Amino Acid Composition and Preparation of High Nutritional Value Food Protein Product, Bulletin of the Georgian Academy of Sciences, 2003,  167,  1, 130-13.
  11. Basilashvili L., Kikvidze M., Bagalishvili M., Nutsubidze N. Selection of the mixed and monocultures of nitrogen fixing microorganisms for production of microbial preparations. Bulletin of the Georgian Academy of Sciences, 2004, 169, 1, 161-164.
  12. Betsiashvili M., Sadunishvili T., Amashukeli N., Tsulukidze N., Shapovalova N., Dzamukashvili N. and Nutsubidze N. Effect of aromatic hydrocarbons on main metabolic and energetic enzymes in maize, ryegrass and kidney bean seedlings. Bulletin of the Georgian Academy of Science, 2004, 170, 1, 172-174.
  13. Pataraya D., Bagalishvili M., Kikvidze M., Basilashvilil L., Gurielidze M. Chigvinadze T. and Nutsubidze N. (). Nitrogenase activity of associativenitrogene fixing microorganisms and their role in Maite growth, development and productivity. JBPC. 2005, 5, 1, 31-35.
  14. Gurielidze M., Berishvili T., Cholokava N., Pataraya D., Nutsubidze N. Chemical composition and microflora of solonchaks and solonetsz soils of eastern Georgia. Proceedings of the Georgian Academy of Sciences,  2007, 5, 2, 57-65.

National Grant

2006 – 2009 – GNSF /ST06/5 – 077 Isolation, characterization of extremophilic actinomycetes from soils of Georgia, the pathways of their application in modern biotechnology.

Pataraya Dodo

1938 - Born
1962 - Tbilisi State University
1969 - Candidate of Biological Sciences
1997 - Doctor of Biological Sciences

Selected Publications

  1. Pataraya D,  Gurielidze M, Nutsubidze N. Biological methods of activity determination of antibiotic compounds produced by actinomycetales.  Buletin of Georgian Acaemy of Sciences, 2000, 161, 3, 535-537.
  2.  Berishvili T,  Pataraya D, Kachniashvili Ch. The isolation of herbicide 2,4-D destructing microorganisms of different taxonomic groups from soils of Georgia. Buletin of Georgian Acaemy of Sciences, 2000, 163, 2, 356-358.
  3. Gurielidze M, Khokhrashvili I, Pataraya D, Devdariani T. Phisico-chemical characterisations of antimicrobial substances produced by Streptomyces sp. 114 strain. Buletin of Georgian Acaemy of Sciences, 2001, 163, 1, 171-173.
  4.  Pataraya D,  Gurielidze M. Biological proprietes of actinomycetes producent antibiotic. Buletin of Georgian Acaemy of Sciences, 2001, 163, 2, 359-360.
  5. Kudukhashvili P.G., Gurielidze M.A. & Pataraya D.T. Study of litic activity of actinomycetes isolated from different soils in Georgia. Applied Biochemistry and Microbiology (in Russian), 2001, 37, 3, 289-291.
  6. Garuchava M.V., Berishvili T.A., Gurielidze M.A., Pataraya D.T., Kakhniashvili Ch. Microbiological degradation of 2,4-D by actinomycetes isolated from different soils of Georgia.   Agrochemistr, 2002. 3, 50-54 (in Russian).
  7.  Berishvili T., Garuchava M., Kakhniashvili Ch., Pataraya D. Physico-chemical characterization and identification of 2,4-dichlorphenoxyacetic acid transformation producrs in soil microorganisms.  Journal of Biological Physics and Chemistry, Basel, Tbilisi,  2002, 2, 1-2, 47-52.
  8. Tsibakhashvili N.Y., Mosulishvili L.M., Kalabegishvili T.L., Pataraya D.T., Gurielidze M.A., Nadareishvili G.S., Holman H-Y.N. Chromate-resistant and reducing-microorganisms in Georgia basalts: their distribution and characterization. Fresenius Environmental Bulletin, 2002, 11, 7, 352-361.
  9. Shengelia N., Pataraya D. Some aspects of microbiological control in wine technology. Science and Technologies, 2004, 10-12. 140-143 (in Georgian).
  10. Berishvili T., Gurielidze M., Kakhniashvili Ch., Pataraya D. A study of the vitality of microorganisms capable   of  degrading 2,4-dichlorophenoxyacetic acid as characterized by NO excretion. Journal of Biological Physics and Chemistry, Basel, Tbilisi, 2004,  4, 4, 181-184.
  11. Pataraya D., Bagalishvili M., Kikvidze M., Basilashvilil L., Gurielidze M. Chigvinadze T. and Nutsubidze N. (). Nitrogenase activity of associativenitrogene fixing microorganisms and their role in Maite growth, development and productivity, JBPC. 2005, 5, 1, 31-35.
  12. Kalabegishvili T., Tsibakhashvili N., Murusidze I., Pataraya D., Gurielidze M. H.-Y. Holman. Formation of Cr(V) and Cr(III) in Arthrobacter oxydans exposed to high concentrations of Cr(VI).  Modern Multidisciplinary Applied Microbiology (Ed. By Mendez-Vilas, A.), Wiley-VCH, Weinhem, 2006, 516-520.
  13.  Tsibakhashvili N., Kalabegishvili T., Rcheulishvili A., Murusidze I., Pataraya D.,  Gurielidze M. H.-Y. Holman, Detoxification of Cr(VI) by basalt-inhabiting bacteria, In Proceedings : “Protection and Restoration of the Environment VIII”, Chania-Crete-Greece, 2006, 115-116.
  14. Gulua L., Patraya D., Gurielidze M., Khutsidze T., Cholokava N., Abutidze M., Salia G. Microflora and antimicrobial activity of green tea extract. Proceedings of the Georgian Academy of Sciences, 2006, 4, 4, 6-9.
  15. Pataraya D., M.Gurielidze, T.Berishvili, N.Cholokava, R.Ckvedelidze, T.Urushadze and E.Kvesitadze. Unusual actinomycetes from various types of soils in Georgia. Journal of Biological Physics and Chemistry,  Basel, Tbilisi, 2006,  6, 4, 149-154.
  16. Gurielidze M., Berishvili T., Cholokava N., Pataraya D., Nutsubidze N. Chemical composition and microflora of solonchaks and solonetsz soils of eastern Georgia. . Proceedings of the Georgian Academy of Sciences, 2007, 5, 2, 57-65.

Grants of the Georgian Academy of Sciences

  1. 1997 – 1999 – 14.2.  Establishment of nitrogen assimilatory enzyme systems and pathways of protein production regulation in leguminous plants
  2. 2000 – 2001 – 14.2.  Effect of nitrogenous microbiological combined fertilizers on regulatory mechanism of nitrogen assimilation processes in leguminous and cereal cultures and growth – development stimulation of plant.
  3. 2002 – 2003 – 14.7.  Correlation between nitrogen – fixing and nitrogen assimilating processes in symbiotic system of leguminous plants.

National Grant

2006 – 2009 – GNSF /ST06/5 – 077 Isolation, characterization of extremophilic actinomycetes from soils of Georgia, the pathways of their application in modern biotechnology.

International Grants

  1. 2004-2006 - CRDF GE-B2-2597. Mechanisms of Microbial Detoxification and Reduction of Heavy Metal Ions( www.crdf.org)
  2. 2006-2008 CRDF_SCCRP, SCI-010002-SC-05. Establishment of a Biotechnological Network of Regional Microbial Culture Collections in the Caucasus (www.crdf.org)

Zaalishvili Giorgi

1947 - Born
1970 – Tbilisi Polytechnical University
1983- Candidate of Biological Sciences
2004 - Doctor of Biological Sciences

Selected Publications

  1. Zaalishvili, G., Khatisashvili, G., Ugrekhelidze D.,Gordeziani, M., Kvesitadze G. Plant potential for detoxification (Review). Applied Biochemistry and Microbiology, 2000, 36, 5, 443-451.
  2. Korte, F., Kvesitadze G., Ugrekhelidze D., Gordeziani, M., Khatisashvili, G. Buadze, O. Zaalishvili, G., Coulston, F. Review: Organic toxicants and plants. Ecotoxicology and Environmental Safety, 2000, 47, 1, 1-26.
  3. Zaalishvili G., Ebelashvili M., Varazashvili T., Pruidze M., Sadunishvili T. Influence of nitrobenzene different concentrations on maize root tip cells ultrastructure results. Biotechnology 2000.  Health Care Agriculture and Food Environmental Biotechnology. ICC-Berlin, 2000, 3-8.
  4. Zaalishvili G., Lomidze E., Buadze E., Sadunishvili T., Tkhelidze P., Kvesitadze G. Electron microscopic investigation of benzidine effect on maize root tip cells ultrastructure, DNA synthesis and calcium homeostasis. International Biodeterioration and Biodegradation, 2000, 46, 133-140.
  5. Gurielidze K.G., Zaalishvili G.G Therapeutic effect during experimental mycosis. Fitoterapia,2000, 140, 430-433.
  6. Varazashvili T., Zaalishvili G., Kurashvili M., Pruidze M., Gordeziani M. Participation of the plant monooxygenase system in adaptation to environmental stress. Journal of Biological Physics and Chemistry, 2001, 1, 38-42.
  7. Varazashvili, T., Khatisashvili, G., Kurashvili, M., Pruidze, M., Ananiashvili, T., Zaalishvili, G., Gordeziani, M. Nitrobenzene oxidizing enzymes in plant cells. Journal of Biological Physics and Chemistry, 2001, 1, 85-88.
  8. Ebelashvili M., Kuprava N., Chkhikvadze Kh, Zaalishvili G. Influence of nitrobenzene different concentration on ultrastructure of maize root tip cells. Bulleten of the Georgia Academy of Sciences, 2002, 165, 2, 373-375.
  9. Zaalishvili G., Sadunishvili T., Scalla R., Laurent F., Kvesitadze G. Electron microscopic investigation of nitrobenzene distribution and effect on plant root tip cells ultrastructure. Ecotoxicology and Environmental Safety, 2002, 52, 190-197.
  10. Kiskeidze, E., Zaalishvili, G., Ebelashvili, M., Khatisashvili, G., Kurashvili, M., Gordeziani, M. Changes of plant monooxygenase system during xenobiotic oxidation. Proceedings of the Georgian Academy of Sciences, 2003, 29, 5-6, 639-644.

International Grants

2004- 2006 ISTC G-1129.Bacteriophage, an effective biological tool against plant diseases caused by pathogenic bacteria (www.istc.ru).

Amashukeli Nanuli

1954 - Born
1976 - Tbilisi State University
1994 - Candidate of Biological Sciences

Selected Publications

  1. Amasukeli N., Shengelia N., Berulava A. Study of various species of barley HPLC. Bulletin of the Georgian Academy of Science, 1995, 152, 4.
  2. Amasukeli N., Shengelia N., Khachidze T. Electrophoretic spectrum and cluster analysis of seed prolamins of Georgina endemic barley species. Bulletin of the Georgian Academy of Science, 1995,152, 4, 96.
  3. Amasukeli N., Khachidze T., Berulava A., Qabzinadze M., Khachidze O. Peptides in seed of barley, wheat and oat. Bulletin of the Georgian Academy of Science, 1995, 152, 3.
  4. Betsiashvili M., Sadunishvili T., Amashukeli N., Kuprava N., Tsulukidze N. Effect of different concentrations of alkanes on maize, ryegrass and kidney bean seedlings. Proceedings of the Georgian Academy of Sciences, 2004,  2, .1-2, 1-5.
  5. Betsiashvili M., Sadunishvili T., Amashukeli N., Tsulukidze N., Shapovalova N., Dzamukashvili N.,  Nutsubidze N. Effect of aromatic hydrocarbons on main metabolic and energetic enzymes in maize, ryegrass and kidney bean seedlings. Bulletin of the Georgian Academy of Science, 2004, 170, 1, 172-174.
  6. Gigolashvili G., Amashukeli N., Sadunishvili T., Betsiashvili M., Tsulukidze N. Establishment of cereal cultures interspecific differences by the method of isoelectrofocusing. Bulletin of the Georgian Academy of Science, 2004, 170, 2, 367-369.
  7. Lomsianidze L., Amashukeli N., Sadunishvili T. The effect of ammonium nitrate doses on amino acid content of proteins in maize grains. Bulletin of the Georgian Academy of Sciences, 2005, 171, 1, 145-147.
  8. Gigolashvili G., Amashukeli N. To the use of protein spectra for making of plant phytoremediatory ability. Frontier research Center for the Global Environment Science, 2005, 7, 445-448.
  9. Lomsianidze L., Amashukeli N., Sadunishvili T. Effect of the introduction of different doses of ammonium nitrate in podzolic soil on maize var. Ajametis tetri seed protein content. Proceedings of the Georgian Academy of Sciences, 2005, 3, 2, 16-20.

Grants of the Georgian Academy of Sciences

2002 – 2003 – 14.7. Correlation between nitrogen – fixing and nitrogen assimilating processes in symbiotic system of leguminous plants.

International Grants

2006-2008. ISTC G-1129. Bacteriophage, an effective biological tool against plant diseases caused by pathogenic bacteria (www.istc.ru).

Gaganidze Dali

1961 - Born
1986 - Tbilisi State University
1991 - Candidate of Biological Sciences

Selected Publications

  1. Odintsova M, Turisheva M, Gaganidze DIn vitro transcriptional activity of chloroplast genome of Azolla pinnata. Plant Physiology ,  1989, 76 2, 120.
  2. Turisheva M, Gaganidze D. et al. Isolation of transcriptionally active DNA-protein complex from chloroplasts of feem Azolla pinnata. Plant Physiology, 1992, 39 1, 55-64.
  3. Turisheva M, Gaganidze D. et al. On physical and chemical characteristics of transcriptionally active DNA-protein complex from chloroplasts of feern Azolla pinata. Plant Physiology, 1993, 41, 77.
  4. Brodzik R, Gaganidze D, Hennig J, Muszynska G, Koprowski H, Sirko A. Transgenic plants as a potential source of an oral vaccine against Helicobacter pylori. In: Food Biotechnology, Bielecki, S., Tramper, J., Polak, J. (Eds) Progress in Biotechnology,  17, Elsevier Science B. 2000,35-42.
  5. Blaszcyk A, Gaganidze D, Sirko A. Using GUS assay to compare the activities of the commonly used constitutive promoters in various organs of transgenic tobacco plants. Cellular and Molecular Biology letters, 2003, 8 3, 50.
  6. Sirko A, Blaszczyk A, Liszewska F, Kazimierczuk K, Gaganidze D.) Genetic engineering of oxidative stress resistance in plants. Sulphur in plants - Abrol, Y.P. and Ahmad, A. (Eds), Kluwer Academic Publishers, Dordrecht, The Netherlands,  2003  245-263.
  7. Liszewska F, Gaganidze D, Sirko A. (Isolation of Nicotiana plumbaginifolia cDNAs encoding isoforms of serine acetyltransferase and O-acetylserine (thiol) lyase in a yeast two-hybrid system with Escherichia coli cysE and cysK genes as baits. Acta Biochim Polon, 2005, 52, 117-128.
  8. Wawrzynska A,  Wawrzynski A, Gaganidze D, Kopera E, Piatek K, Bal W, Sirko A. Overexpression of genes involved in phytochelatin biosynthesis in Escherichia coli: effects on growth, cadmium accumulation and thiol level. Acta Biochim Polon ,2005, 52, 109-116.

International Grants

  1. 2003-2004, 5th Framework Programme of European Union "Optimising nutritional quality of crops (OPTI-2); QLRT-2000-00103 and QLRT-2001-02928 [NAS]
  2. 2003-VI. 2005, 5th Framework Programme of European Union "Development of systems to improve phytoremediation of metal contaminated soils through improved phytoaccumulation" (PHYTAC); QLRT-2001-00429 and QLRT- 2001-02778 [NAS]
  3. 2002-2004, POLONIUM-Polish-French bilateral program.  Obtaining of antibodies of protein UreB of Helicobacter pylori by immunization of goose.
  4. 2001-2004, KBN (6P04A07121). Metabolism of sulfur in plants and ways of its regulation.
  5. 1999-02.2003, USDA Scientific Cooperation Program "Development of Techniques for Agriculturally Valuable Crops to Create Edible Vaccines"
  6. 1997-2000, KBN (6P04B01112). Obtaining of transgenic plants producers of Helicobacter pylori recombinated urease producers.
  7. 1996-1997, NATO. Development of Collaborative Research "Construction of transgenic plants producing immunogenic protein of therapeutic significance"

Betsiashvili Mariam

1973 - Born
1995 – Georgian Politechnical Universitety
2003 - Candidate of Biological Sciences

Selected Publications

  1. Pataraia D. Basilashvili L., Bagalishvili M., Kikvidze M., Betsiashvili M., Nutsubidze N. Influence of nodule bacteria and different microorganisms on soybean growth. Bulletin of the Georgian Academy of Sciences, 1999, 160, 2, 334-336.
  2. Kuprava N., Betsiashvili M., Sadunishvili T., Nutsubidze N. Ammonia Assimilation in Soya Seedlings of Different Varieties, Bulletin of the Georgian Academy of Sciences, 2000, 162,  3,  549-551.
  3. Betsiashvili M., Kuprava N., Sadunishvili T., Nutsubidze N. Study of the Georgian Soybean Varieties for Preparation of Valuable Food Protein. Bulletin of the Georgian Academy of Sciences, 2002, 165, 2, 358-360.
  4. Betsiashvili M., Sadunishvili T., Gigolashvili G., Nutsubidze N., Kvesitadze G. Valuable food protein preparation from soybean. Advances in Food Sciences, 2002, 24, 1, 20-23.
  5. Betsiashvili M., Kuprava N., Sadunishvili T., Nutsubidze N. Study of the Soybean Protein Amino Acid Composition and Preparation of High Nutritional Value Food Protein Product, Bulletin of the Georgian Academy of Sciences, 2003,  167, 1, 130-133.
  6. Betsiashvili M., Sadunishvili T., Amashukeli N., Kuprava N., Tsulukidze N. Effect of different concentrations of alkanes on maize, ryegrass and kidney bean seedlings. Proceedings of the Georgian Academy of Sciences, 2004, 2, .1-2, 1-5.
  7. Betsiashvili M., Sadunishvili T., Amashukeli N., Tsulukidze N., Shapovalova N., Dzamukashvili N. and Nutsubidze N. Effect of aromatic hydrocarbons on main metabolic and energetic enzymes in maize, ryegrass and kidney bean seedlings. Bulletin of the Georgian Academy of Science, 2004, 170, 1,  172-174.
  8. Gigolashvili G., Amashukeli N., Sadunishvili T., Betsiashvili M., Tsulukidze N. Establishment of cereal cultures interspectic differences by the method of isoelectrofocusing. Bulletin of the Georgian Academy of Science, 2004, 170,  2,  367-369.
  9. Betsiashvili M., Sadunishvili T., Nutsubidze N., Kuprava N. The effect of different concentration of hydrocarbons on main metabolic and energetic processes of plants. Frontier Research Center for the Global Enviroment Science (GESC), 2005, 7,  441-444.
  10. Kuprava N., Betsiashvili M., Dzamukashvili N. Sadunishvili T., Nutsubidze N. Influence of Rhizobium and free-living nitrogen-fixsing bacteria on nitrogen assimilation enzymes of soybean plant. Bulletin of the Georgian Academy of Sciences, 2006, 173 , 2,  348-351.
  11. Sadunishvili T., Kuprava N., Aplakov V., Betsiashvili M., Zaalishvili G., Mitaishvili T., Kvesitadze E. Ammonia Assimilation Enzymes and cell ultrastructure in Maize and Soybean. Proceedings of the Georgian Academy of Sciences, 2006, l4  2,  16-22.
  12. Tsulukidze N., Betsiashvili M., Dzamukashvili N., Sadunishvili T., Kvesitadze E. The impact of bioactive preparations on the resistance of reygrass exposed to organic tocants – benzene, 3, 4 –benzpyrene and trinitrotoluene. Proceedings of the Georgian Academy of Sciences, 2007, 5, 1, 1-7.

Grants of the Georgian Academy of Sciences

  1. 2000 – 2001 – 14.2.  Effect of nitrogenous microbiological combined fertilizers on regulatory mechanism of nitrogen assimilation processes in leguminous and cereal cultures and growth – development stimulation of plant.
  2. 2002 – 2003 – 14.7 Correlation between nitrogen – fixing and nitrogen assimilating processes in symbiotic system of leguminous plants.

International Grants

  1. 2004- 2006 ISTC G-1129. Bacteriophage, an effective biological tool against plant diseases caused by pathogenic bacteria (www.istc.ru)
  2. 2002-2005. ISTC G-718 Elaboration of a new strategy of phytoremediation and long-term protection of the environment polluted by hydrocarbons (www.istc.ru)
  3. 2004-2006. STCU P-196. Microbial diversity for novel biotechnology applications (www.stcu.int)
  4. 2006-2008 CRDF_SCCRP, SCI-010002-SC-05. Establishment of a Biotechnological Network of Regional Microbial Culture Collections in the Caucasus(www.crdf.org).
  5. 2006-2009. STCU 3802 Novel approach for the improvement of ecological guarantee of oil pipelines (www.stcu.int)

Gurielidze Manana

1967 - Born
1991 - Tbilisi State University
2001 - Candidate of Biological Sciences

Selected Publications

  1. Gurielidze M., D.Pataraya, G.Kvesitadze. Isolation of strains of actinomycetes active to phytopathogenic fungi from  rhizosphere and plant roots. Bulletin of the Georgian Academy of Sciences, 1999, 160, 3,  559-561.
  2. Pataraya D., M.Gurielidze, N.Nutsubidze.Biological method of activity determination of antibiotic compounds produced by actinomycetes. Bulletin of the Georgian Academy of Sciences ,2000, 161, 3,  535-537.
  3. Gurielidze M., I.Khokhashvili, D.Pataraya, T.Devdariani. Physico-chemical characteristics of antimicrobial substances produced by Streptomyces sp.114 strain . Bulletin of the Georgian Academy of Sciences, 2001,163, .1, 171-173.
  4. Pataraya D., M.Gurielidze. “Biological properties of antibiotic producent actinomycetes”. Bulletin of the Georgian Academy of Sciences ,2001, 163, 2, 198-199.
  5. Kudukhashvili P., Gurielidze M., Pataraya D.  Study of the lytic activities of actinomycetes isolation from different soils of Georgia. Applied Biochemistry and Microbiology,  37, 3,2001, 289-291 (in Russian).
  6. Berishvili T., Gurielidze M., Garuchava M., Pataraya D., Kakhniashvili Ch. Biological characteristics of actinomycetes with degrading ability for pheno[yacetic acid-derived herbicides.  Bulletin of the Georgian Academy of Sciences ,2001, 165, 1, 122-124.
  7. Garuchava M.V. T.A.Berishvili, M.Gurielidze, D.Pataraya, Ch. Kakhniashvili.Microbiological degradation od 2,4-D by actinomycetes isolated from different soils of Georgia . Agrochemistry, 2002,3, 50-54 (in Russian).
  8. Tsibakhashvili N.Y., Mosulishvili L., Kalabegishvili T., Pataraya D., Gurielidze M., Nadareishvili G., H-Y.N.Holman. Chromate-resistant and reducing-microorganisms in Georgia basalts: their distribution and characterization .Fresenius Environmental Bulletin, 2002, 11(7):352-361.
  9. Gulua L., Patraya D., Gurielidze M., Khutsidze T., Cholokava N., Abutidze M., Salia G. Microflora and antimicrobial activity of green tea extract. Proceedings of the Georgian Academy of Sciences, 2006, 4, 4, 6-9.
  10. Pataraya D., M.Gurielidze, T.Berishvili, N.Cholokava, R.Ckvedelidze, T.Urushadze and E.Kvesitadze. Unusual actinomycetes from various types of soils in Georgia. Journal of Biological Physics and Chemistry, Basel, Tbilisi. 2006,  6, 4, 149-154.
  11. Gurielidze M., Berishvili T., Cholokava N., Pataraya D., Nutsubidze N. Chemical composition and microflora of solonchaks and solonetsz soils of eastern Georgia. Proceedings of the Georgian Academy of Sciences, 2007, 5, 2, 57-65.
  12. Bagalishvili M., Basilashvili L., Kikvidze M., Gurielidze M., Nutsubidze N.           Determination of antimicrobial activity of nitrogen fixing bacteria. Bagalishvili M., Basilashvili L., Kikvidze M., Gurielidze M., Nutsubidze N. Determination of antimicrobial activity of nitrogen fixing bacteria. Proceedings of the Georgian Academy of Sciences, Biol. Ser.B. 2007, 5, 1, 45-48.

Grant of the Georgian Academy of Sciences

2002 – 2003 – 14.7 Correlation between nitrogen – fixing and nitrogen assimilating processes in symbiotic system of leguminous plants.

National Grant

2006 – 2009 – GNSF /ST06/5 – 077   Isolation, characterization of extremophilic actyinomycetes from soils of Georgia, the pathways of their application in modern biotechnology.

International Grant

2000-2002 - ISTC G-284 Biotransformation of carcinogens for the protection of the environment and human health (www.stcu.int).

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