Search: 
  

Labaratory of Biological Oxidation

Head of laboratory, Doctor, ProfessorM. Gordeziani

Main Directions

Plant xenobiochemistry – Establishment of regulatory mechanisms of exogenous organic toxicants detoxification in plant cell. Investigation of plant enzymes (cytochrome P450-containing monooxygenases, peroxidases, phenoloxidases, nitroreductases, dehalogenases) participating in degradation of organic toxicants.
Investigation of biochemical principles of phytoremediation of contaminated by chemical toxicants environment (air, water, soil).
Elaboration of biochemical criteria for selection of plants for phytoremediation.
Study of plant adaptation and immunochemical mechanisms to environment stress-factors.

Research Activity

The xenobiotics oxidation by plant cytochrome P450-containing monooxygenase system has been studied. It has been revealed that in plant cells the monooxygenase system exists in soluble and membrane-bound forms.
It was stated that the same fund of cytochrome P450-containing monooxygenase system must participate in plant intracellular metabolic (e.g. biosynthesis of phenolic compounds) and detoxification processes. It was shown that xenobiotic hydrophobicity is the main factor which determines the quantity of cytochrome P450 switching from biosynthesis to detoxification.
It was shown that efficiency of cytochrome P450 induction by xenobiotics depends on the chemical nature of intermediates formed during inductor oxidation, particularly, the higher acivity of intermediate the lower cytochrome P450 induction. Under certain conditions (e.g. plant aging, aeration, action of radical intermediates) plant cytochrome P450 loses NADPH-dependent oxidation ability and catalyzes oxidation reactions in the presence of H2O2, i.e. monooxygenase mechanism of xenobiotic oxidation is changed by peroxidase one.
The close linkage between detoxification and cell energetical processes has been revealed. It has been shown that in the respiratory chain of mitochondria in conditions of electrons free transport the electrons are used for detoxification of xenobiotics penetrated into the cell . In these conditions migration of electrons from mitochondrial respiratory chain complex III to cytochrome b5 of microsomal redox-chain takes place. When the monooxygenase system does not participate in detoxification process, the initial components of monooxygenase system (NADPH cytochrome P450 reductase and cytochrome b5) transfer the reducing equivalents to mitochondrial bc1-complex. Existence of this mechanism stipulates insertion of NADPH reducing equivalents besides NADH in cell energetics which is very important for the plant with a rich source of NADPH via photosynthesis.
The enzymatic transformation of 2,4,6-trinitrotoulene (TNT) is mainly performed by NAD(P)H-nonspecific nitroreductase acting on the TNT nitro groups. Nitroreductase activity was revealed in root cell cytosol and expression was strongly induced by plant cultivation on TNT-containing media. A plant’s nitroreductase activity and its ability to uptake TNT from aqueous solutions were correlated. These results suggest that the plant nitroreductase activity may serve as a biochemical criteria to select plants for phytoremediation of soils contaminated with TNT.
According to the laboratory conception, the xenobiotic oxidation in plant proceeds via the interchange of oxidative enzymes (basically cytochrome P450-containing monooxygenase, peroxidase, phenoloxidase). We suppose that plant cytochrome P450 plays an important role in plant adaptation to stress factors. Currently the works are being carried out to corroborate this hypothesis experimentally.

Members

  1. Gordeziani Marlen – Head of laboratory, corresponding-member of Georgian Academy of Sciences, Doctor, professor
  2. Khatisashvili Gia – Doctor , chief scientist
  3. Varazi Tamar – PhD, scientist
  4. Kurashvili Maritsa – PhD, scientist
  5. Pruidze Marina –PhD ,scientist
  6. Adamia George – PhD,lab assistant
  7. Ananiashvili Tamar –PhD, lab assistant

Gordeziani Marlen

1938 – Born
1962 - Tbilisi Zoo-technical-Veterinary Institute
1975 - Candidate of Biological Sciences
1992 – Doctor of Biological Sciences
1997 - Corr. member of the Georgian Academy of Sciences
1979 - Head of Laboratory
2004 - Premium by Durmishidze of the Georgian Academy of Sciences

Selected Publications

  1. Gordeziani M. Cytochrome P450 in plants. Proceedings of the Georgian Academy of Sciences, Biological Series, 1987, 13, 3, 177–186.
  2. Gordeziani M., Khatisashvili G., Adamia G. The cytochrome P450-containing monooxygenase system of plant membranes. Works of VI International Conference: “Biological Membranes in Norm and Pathology”, Georgia, Tbilisi, 1989, 41–43.
  3. Khatisashvili G., Gordeziani M., Lomidze E., Adamia G. NADPH redox-cycle at oxygen deficite in cell-free plant systems. Abstracts of V International Conference: “Cytochrome P-450 and Modification of Macromolecules”, Ukraine, Yalta, 1989, 156.
  4. Togonidze L., Khatisashvili G., Kobakhidze L., and Gordeziani M. NAD(P)H-Dependent fractional oxidation of dimethylaniline in plant leaves. Bulletin of the Georgian Academy of Sciences, 1990, 140, 125–128.
  5. Khatisashvili G., Gordeziani M. Hydroxylation of xenobiotics by plant cytochrom P-450. Proceedings of VII International Conference: "Biochemistry and Biophysics of Cytochrome P-450; Structure and Function, Biotechnological and Ecological Aspects". Moscow, Russia, 1991, 546–548.
  6. Gordeziani M., Khatisashvili G., Kvesitadze G. Free and coupled with hydroxylation of xenobiotics oxidation of NADPH. Doklady AN SSSR, 1991, 320, 2, 417–420.
  7. Gordeziani M., Khatisashvili G., Kurashvili M. Distribution of NADPH-cytochrome P-450-reductase in plant cell. Bulletin of the Georgian Academy of Sciences, 1991, 143, 321–324.
  8. Khatisashvili G., Gordeziani M., Kurashvili M. Cytochromal components of plant microsomes and possibility of their induction. Bulletin of the Georgian Academy of Sciences, 1991, 143, 183–192.
  9. Balashvili M., Gordeziani M., Khatisashvili G., Papelishvili R., Jokhadze D. Removal of xenobiotic inhibitory effect by acceleration of their hydroxylation of protein and RNA-synthesizing activity in plants. Proceedings of the Georgian Academy of Sciences, Biological Series, 1992, 18, 5, 303–310.
  10. Khatisashvili G., Kurashvili M., Gordeziani M., Kvesitadze G. Monooxygenase and peroxydase pathways of xenobiotics detoxication in higher plants. Fresenius Environmental Bulletin, 1993, 2, 103–108.
  11. Khatisashvili G., Kurashvili M., Chkhikvishvili I., Gordeziani M., Kvesitadze G. Transformation of monooxygenase mechanism into peroxidase one and microsomal oxidation of flavonoids in plants. Proceedings of the Georgian Academy of Sciences, Biological Series, 1993, 19, 318–323.
  12. Khatisashvili G., Kurashvili M., Gordeziani M., Kvesitadze G. Functional evolution of separate components of plant monooxygenase system involved in xenobiotic detoxication. Fresenius Environmental Bulletin, 1994, 3, 621–626.
  13. Khatisashvili G., Kurashvili M., Gordeziani M. Isolation of plant microsomal fraction and characterization of its oxidative systems. Bulletin of the Georgian Academy of Sciences, 1995, 152, 818–824.
  14. Khatisashvili G., Gordeziani M., Kvesitadze G., Korte F. Plant monooxygenases: participation in xenobiotic oxidation. Ecotoxicology and Environmental Safety, 1997, 36, 118–122.
  15. Zaalishvili G., Khatisashvili, G., Ugrekhelidze D., Gordeziani M., Kvesitadze G. Plant potential for detoxification (Review). Applied Biochemistry and Microbiology, 2000, 36, 5, 443–451.
  16. Gogoberidze M., Zaalishvili G., Khatisashvili G., Ugrekhelidze D., Gordeziani M., Kvesitadze G. Bioremediation – Contemporary strategy of xenobiotics’ detoxification. Contaminated Soil Proceedings of the 7th International FZK/TNO Conference, 2000, 1, 397–404.
  17. Varazashvili T., Kurashvili M., Pruidze M., Gordeziani M. About participation of monooxygenase system in the processes of plant adaptation. The Problems of Agrarian Scienc, Scientific Works, IX, 2000, 110–111.
  18. Kiskeidze E., Khatisashvili G., Gordeziani M. Changes of plant monooxygenase system while xenobiotics oxidation. The Problems of Agrarian Science, Scientific Works, IX, 2000, 115–118.
  19. Ananiashvili, T., Khatisashvili, G., Gordeziani, M. About energetic significance of initial components of plant monooxygenase system. The Problems of Agrarian Science, Scientific Works, IX, 2000, 119-123.
  20. Khatisashvili G., Gordeziani M., Varazashvili T., Pruidze M. The Role of oxidative enzyme systems in the process of phytoremediation. Contaminated Soils, Sediments and Water, London, UK. 2001, August 14–17.
  21. Kvesitadze G., Gordeziani M., Khatisashvili G., Sadunishvili T., Ramsden J.J. Some aspects of the enzymatic basis of phytoremediation. Journal of Biological Physics and Chemistry, 2001, 1, 2, 49–57.
  22. Varazashvili T., Kurashvili M., Pruidze M., Gordeziani M. Monooxygenase system participation in the processes of plant adaptation. Proceedings of the Georgian Academy of Sciences, Biological Series A, 2001, 27, 33–37.
  23. Varazashvili T., Khatisashvili G., Kurashvili M., Pruidze M., Ananiashvili T., Zaalishvili G., Gordeziani M. Nitrobenzene oxidizing enzymes in plant cells. Journal of Biological Physics and Chemistry, 2001, 1, 85–88.
  24. Varazashvili T., Zaalishvili G., Kurashvili M., Pruidze M., Gordeziani M. Participation of the plant monooxygenase system in adaptation to environmental stress. Journal of Biological Physics and Chemistry, 2001, 1, 38–42.
  25. Varazashvili T., Khatisashvili G., Kurashvili M., Pruidze M., Ananiashvili T., Zaalishvili G., Gordeziani M. Nitrobenzene oxidizing enzymes in plant cells. Journal of Biological Physics and Chemistry, 2001, 1, 85–88.
  26. Kiskeidze E., Zaalishvili G., Ebelashvili, M., Khatisashvili G., Kurashvili M., Gordeziani M. Changes of plant monooxygenase system during xenobiotic oxidation. Proceedings of the Georgian Academy of Sciences, Biological Series A, 2003, 29, 5-6, 639–644.
  27. Kurashvili M., Pruidze M., Kiskeidze E., Varazashvili T., Ananiashvili T., Khatisashvili G., Gordeziani M. Influence of different factors on nitrobenzene oxidation in the plant cell. Journal of Biological Physics and Chemistry, 2003, 3, 2, 45–49.
  28. Adamia G., Khatisashvili G., Varazashvili T., Ananiashvili T., Gvakharia V., Adamia T., Gordeziani M. Determination of the type and rate of soil contamination with heavy metals and organic toxicants on the territories of Military Proving Grounds in Georgia. Bulletin of the Georgian Academy of Sciences, 2003, 167, 1, 155–158.
  29. Adamia G., Varazashvili T., Ananiashvili T., Kurashvili M., Pruidze M., Gvakharia V., Machitadze N., Gordeziani M. Study of lead accumulation ability of some sowing plants. Bulletin of the Georgian Academy of Sciences, 2005, 171, 3, 592–595.
  30. Kurashvili M., Varazi T., Ananiashvili T., Gordeziani M. Participation of plant oxidizing enzymes in hydroxylation of the hydrocarbons. International Conference “Plant and Microbial Enzymes: Isolation, Characterization and Biotechnology Applications”, Tbilisi, Georgia, 2-5th July ,2007, 60-63.

Monographs

  1. Gaprindashvili, I., Gordeziani, K., Gordeziani, M. Light, Heat, Soil, Plant. Tbilisi, 1969, 221.
  2. Gordeziani, M., Gordeziani, K. Biophysical chemistry and Agriculture. Tbilisi, 1971, 207.
  3. Gordeziani, M., Kvesitadze G. Chemical Basis of Ecology (Ed. D.Ugrekhelidze) Tbilisi, 2000, 311.
  4. Gordeziani, M., Khatisashvili, G., Kirtadze, E. Bioenergetic and Regulation of Oxidative Processes. (Ed. G. Kvesitadze) Tbilisi, 2004, 245.
  5. Gordeziani, M., Khatisashvili, G. Membranous Xenobiochemistry. (Ed. G. Kvesitadze) Tbilisi, 2005, 281.
  6. Gordeziani, M., Varazi T., Kurashvili, M., Pruidze, M. Biological Oxidation and Chemiosmosis. (Ed. N. Nutsubidze) Tbilisi, 2007, 282.

Grants of the Georgian Academy of Sciences

  1. 1995-1996. Investigation of biochemical function of cytochrome P450 containing monooxygenases in plant resistance.
  2. 1997-1999 14.1. Study of molecular mechanisms of organic xenobiotic detoxification in lower and higher plants.
  3. 2000-2001 14.6. Enhancement of plant detoxification ability by stress-factores as main element of phytoremediation.
  4. 2002-2003 14.6.02. Regulation of plant oxidative systems participating in organic xenobiotic detoxification.
  5. 2004-2005 14.5.04. Detoxification of environmental pollutants (organic toxicants) by plant enzymatic systems.

International Grants

  1. 1999-2001 – INTAS-Georgia 97-0716 "Coordination of oxidative enzymes as a key factor in detoxification of organic xenobiotics in plants" (www.intas.be)
  2. 2001-2004 – ISTC G369 "Elaboration of methods of bioremediation of contaminated soils on former military locations and proving grounds in Georgia" (www.istc.ru)
  3. 2002-2005 – ISTC G718 "Elaboration of a new strategy of phytoremediation and long-term protection of the environment polluted by hydrocarbons" (www.istc.ru)
  4. 2007-2010 – ISTC G1408 "Creation of a Novel Complex Phytoremediation Technology for Rehabilitation of Soils and Waters Polluted with Explosives" (www.istc.ru)

Khatisashvili Gia

1963 - Born
1984 - Tbilisi State University
1991 - Candidate of Biological Sciences
1999 - Doctor of Biological Sciences

Stipends

  • 1997 - President stipend (II degree)
  • 2001-2003 - President stipend (I degree)

Selected Publications

  1. Togonidze L., Khatisashvili G., Kobakhidze L., Gordeziani M. NAD(P)H-Dependent fractional oxidation of dimethylaniline in plant leaves. Bulletin of the Georgian Academy of Sciences, 1990, 140, 125–128.
  2. Khatisashvili G., Gordeziani M. Hydroxylation of xenobiotics by plant cytochrom P-450. Proceedings of VII International Conference: "Biochemistry and Biophysics of Cytochrome P-450; Structure and Function, Biotechnological and Ecological Aspects". Moscow, Russia, 1991, 546–548.
  3. Gordeziani M., Khatisashvili G., Kvesitadze G. Free and coupled with hydroxylation of xenobiotics oxidation of NADPH. Doklady AN SSSR. 1991, 320, 2, 417–420.
  4. Gordeziani M., Khatisashvili G., Kurashvili M. Distribution of NADPH-cytochrome P-450-reductase in plant cell. Bulletin of the Georgian Academy of Sciences, 1991, 143, 321–324.
  5. Khatisashvili G., Gordeziani M., Kurashvili M. Cytochromal components of plant microsomes and possibility of their induction. Bulletin of the Georgian Academy of Sciences, 1991, 143, 183–192.
  6. Balashvili M., Gordeziani M., Khatisashvili G., Papelishvili R., Jokhadze D. Removal of xenobiotic inhibitory effect by acceleration of their hydroxylation of protein and RNA-synthesizing activity in plants. Proceedings of the Georgian Academy of Sciences, Biological Series, 1992, 18, 5, 303–310.
  7. Khatisashvili G., Kurashvili M., Gordeziani M., Kvesitadze G. Functional evolution of separate components of plant monooxygenase system involved in xenobiotic detoxication. Fresenius Environmental Bulletin, 1994, 3, 621–626.
  8. Khatisashvili G., Kurashvili M., Gordeziani M., Kvesitadze G. Monooxygenase and peroxydase pathways of xenobiotics detoxication in higher plants. Fresenius Environmental Bulletin, 1993, 2, 103–108.
  9. Khatisashvili G., Kurashvili M., Chkhikvishvili I., Gordeziani M., Kvesitadze G. Transformation of monooxygenase mechanism into peroxidase one and microsomal oxidation of flavonoids in plants. Proceedings of the Georgian Academy of Sciences, Biological Series, 1993, 19, 318–323.
  10. Khatisashvili G., Kurashvili M., Gordeziani M. Isolation of plant microsomal fraction and characterization of its oxidative systems. Bulletin of the Georgian Academy of Sciences, 1995, 152, 818–824.
  11. Gordeziani M., Khatisashvili G., Ananiashvili T., Varazashvili T., Kurashvili M., Kvesitadze G., Tkhelidze P. Energetic significance of plant monooxygenase individual components participating in xenobiotic degradation. International Biodeterioration and Biodegradation, 1999, 44, 49–54.
  12. Khatisashvili G., Gordeziani M., Kvesitadze G., Korte F. Plant monooxygenases: participation in xenobiotic oxidation. Ecotoxicology and Environmental Safety, 1997, 36, 118–122.
  13. Gogoberidze M., Zaalishvili G., Khatisashvili G., Ugrekhelidze D., Gordeziani M., Kvesitadze G. Bioremediation – contemporary strategy of xenobiotics’ detoxification. Contaminated Soil . Proceedings of the 7th International FZK/TNO Conference, 2000, 1, 397–404.
  14. Korte F., Kvesitadze G., Ugrekhelidze D., Gordeziani M., Khatisashvili G., Buadze O., Zaalishvili G., Coulston F. Review: Organic toxicants and plants. Ecotoxicology and Environmental Safety, 2000, 47, 1, 1–26.
  15. Kiskeidze E., Khatisashvili G., Kurashvili M., Shavgulidze G. Action of lipid peroxidation on monooxygenase System participating in xenobiotic detoxification. Bulletin of the Georgian Academy of Sciences, 2000, 162, 1,156–158.
  16. Ananiashvili T., Khatisashvili G., Gordeziani M. About energetic significance of initial components of plant monooxygenase system. The Problems of Agrarian Science, Scientific Works, IX, 2000, 119-123.
  17. Zaalishvili G., Khatisashvili G., Ugrekhelidze D., Gordeziani M., Kvesitadze G. Plant potential for detoxification (Review). Applied Biochemistry and Microbiology, 2000, 36, 5, 443–451.
  18. Khatisashvili G., Gordeziani M., Varazashvili T., Pruidze M. The role of oxidative enzyme systems in the process of phytoremediation. Contaminated Soils, Sediments and Water. London, UK. 2001, August 14–17.
  19. Kvesitadze G., Gordeziani M., Khatisashvili G., Sadunishvili T., Ramsden J.J. Some aspects of the enzymatic basis of phytoremediation. Journal of Biological Physics and Chemistry, 2001, 1, 2, 49–57.
  20. Varazashvili T., Khatisashvili G., Kurashvili M., Pruidze M., Ananiashvili T., Zaalishvili G., Gordeziani M. Nitrobenzene oxidizing enzymes in plant cells. Journal of Biological Physics and Chemistry, 2001, 1, 85–88.
  21. Varazashvili T., Khatisashvili G., Kurashvili M., Pruidze M., Ananiashvili T., Zaalishvili, G., Gordeziani, M. Nitrobenzene oxidizing enzymes in plant cells. Journal of Biological Physics and Chemistry, 2001, 1, 85–88.
  22. Khatisashvili, G., Adamia, G., Pruidze, M., Kurashvili, M., Varazashvili, T., Ananiashvili T. TNT uptake and nitroreductase activity of plants. 8th International FZK/TNO Conference on Contaminated Soil. ConSoil 2003, Gent, Belgium, 2-16 May 2003, 2482–2485.
  23. Kiskeidze E., Zaalishvili G., Ebelashvili M., Khatisashvili G., Kurashvili M., Gordeziani M. Changes of plant monooxygenase system during xenobiotic oxidation. Proceedings of the Georgian Academy of Sciences, Biological Series A, 2003, 29, 5-6, 639–644.
  24. Kuchukashvili Z., Davitaia G., Gorozia I., Kvesitadze G., Lomidze E. and Khatisashvili G. Antioxidant characteristics of phenolic compounds of Satureia hortensis, 2003, 3, 3/4. 85–88.
  25. Kurashvili M., Pruidze M., Kiskeidze E., Varazashvili T., Ananiashvili T., Khatisashvili G., Gordeziani M. Influence of different factors on nitrobenzene oxidation in the plant cell. Journal of Biological Physics and Chemistry, 2003, 3, 2, 45–49.
  26. Kuchukashvili Z., Davitaia G., Avaliani N., Lomidze E., Khatisashvili G., Arziani B. The influence of prostaglandins on oxidative metabolism of 3,4-benzpyrene in rat liver cell microsomes. Bulletin of the Georgian Academy of Sciences, 2003, 168, 1, 101–104.
  27. Adamia G., Khatisashvili G., Varazashvili T., Ananiashvili T., Gvakharia V., Adamia T., Gordeziani M. Determination of the type and rate of soil contamination with heavy metals and organic toxicants on the territories of military proving grounds in Georgia. Bulletin of the Georgian Academy of Sciences, 2003, 167, 1, 155–158.
  28. Pruidze M.V., Khatisashvili G.A., Omiadze N.T. Oxidation of organic xenobiotics by phenoloxidase from tea leaves. Annals of Agrarian Science, 2006, 4, 2, 69–72.
  29. Best E.P.H., Kvesitadze G., Khatisashvili G., Sadunishvili T. Plant processes important for the transformation and degradation of explosives contaminants. Zeitschrift fur Naturforschung, 2005, 60, 340–348.
  30. Zaalishvili G., Varazashvili T., Ananiashvili T., Khatisashvili G., Kvesitadze E. Ultrastructural Reorganization of plant cell in the process of xenobiotics metabolism. Annals of Agrarian Science, 2005, 3, 3, 117–122.
  31. Khatisashvili G., Kvesitadze G., Adamia G., Gagelidze N., Sulamanidze L., Ugrekhelidze D., Zaalishvili G., Ghoghoberidze M., Ramishvili M. Bioremediation of contaminated soils on the former military locations and proving grounds in Georgia. Journal of Biological Physics and Chemistry, 2004, 4, 3, 162–168.
  32. Kvesitadze G., Best E.P.H., Khatisashvili G., Sadunishvili T. Plant process important for the transformation and degradation of explosives contaminants. In: Phytoremediation: environmental and molecular biological aspects. OECD workshop, Hungary, Abstracts, 2004, 55.
  33. Tinikashvili L., Varsimashvili K., Gagelidze N., Amiranashvili L., Chrikishvili D., Kirtadze E., Khatisashvili G., Ghoghoberidze M. Influence of temperature on growth and degradation ability of microorganisms capable for degradation of 2,4,6-trinitrotoluene and mineral oil. Proceedings of the Georgian Academy of Sciences, Biological Series A, 2004, 30, 4, 493–497.
  34. Varsimashvili Kh., Tinikashvili L., Amiranashvili L., Gagelidze N., Kirtadze E., Khatisashvili G., Ghoghoberidze M. Influence of some physicochemical factors in different microorganisms capable for degradation of 2,4,6-trinitrotoluene and mineral oil. Proceedings of the Georgian Academy of Sciences, Biological Series B, 2004, 2, 3-4, 104–109.
  35. Adamia G., Ghoghoberidze M., Graves D., Khatisashvili G., Kvesitadze G., Lomidze E., Ugrekhelidze, D., Zaalishvili, G. Absorption, distribution and transformation of TNT in higher plants. Ecotoxicology and Environmental Safety, 2006, 64, 136–145.

Monographs

  1. Gordeziani, M., Khatisashvili, G., Kirtadze, E. Bioenergetic and Regulation of Oxidative Processes (Ed. G. Kvesitadze), Tbilisi, 2004, 245.
  2. Kvesitadze, G., Khatisashvili, G., Sadunishvili, T., Evstigneeva, Z. Metabolism of Anthropogenic Toxicants in Higher Plants. Moscow, Nauka, 2005, 119.
  3. Papunidze, V., Khatisashvili, G., Sadunishvili, T. Plant for a Healthy Environment (Ed. G. Kvesitadze) Batumi, Ajara, 2005, 229.
  4. Gordeziani M., Khatisashvili, G. Membranous Xenobiochemistry (Ed. G. Kvesitadze) Tbilisi, 2005, 281.
  5. Kvesitadze G., Khatisashvili G., Sadunishvili T., Ramsden J.J. Biochemical Mechanisms of Detoxification: Basis of Phytoremediation. Berlin, Heidelberg, Springer, 2006, 262.

Grants of the Georgian Academy of Sciences

  1. 1995-1996. Investigation of biochemical function of cytochrome P450 containing monooxygenases in plant resistance.
  2. 1997-1999 14.1. Study of molecular mechanisms of organic xenobiotic detoxification in lower and higher plants.
  3. 2000-2001 14.6. Enhancement of plant detoxification ability by stress-factores as main element of phytoremediation.
  4. 2002-2003 14.6.02. Regulation of plant oxidative systems participating in organic xenobiotic detoxification.
  5. 2004-2005 14.5.04. Detoxification of environmental pollutants (organic toxicants) by plant enzymatic systems.

International Grants

  1. 1999-2001 – INTAS-Georgia 97-0716 "Coordination of oxidative enzymes as a key factor in detoxification of organic xenobiotics in plants" (www.intas.be)
  2. 2001-2004 – ISTC G369 "Elaboration of methods of bioremediation of contaminated soils on former military locations and proving grounds in Georgia" (www.istc.ru)
  3. 2002-2005 – ISTC G718 "Elaboration of a new strategy of phytoremediation and long-term protection of the environment polluted by hydrocarbons" (www.istc.ru)
  4. 2006-2008 – STCU 3802 "Novel approach for the improvement of ecological garantee of oil pipelines " (www.stcu.int)
  5. 2007-2010 – ISTC G1408 "Creation of a Novel Complex Phytoremediation Technology for Rehabilitation of Soils and Waters Polluted with Explosives" (www.istc.ru)
© 2007-2008 Durmishidze Institute of Biochemistry and Biotechnology (DIBB).
All rights reserved.